58P Quantitative indicators of TREC and KREC excision rings in breast cancer

The excisional rings of the T cell receptor rearrangement (TREC) and κ-deletion element (KREC) are extrachromosomal DNA structures formed during V(D)J-recombination. A decrease in number TREC KREC below age-related values may be a manifestation immunodeficiency conditions, which can caused by oncological hematological diseases. Objective: To study change amount breast cancer. Methods. For study, blood was taken from patients with malignant neoplasms main group healthy women various ages. Of these 35 individuals 77 Median age 54 years (Q1-Q3: 36-81 years). Quantification performed real-time PCR using IMMUNO-BIT reagent kit (ABV-test LLC) accordance instructions for kit. extraction whole AmpliPrime RIBO-prep (NextBio LLC). According to our individuals, level is 75.6/105 PBMC 19.2-135.3), 317.3/105 118.1-565.9). Whereas cancer, 4.4/105 0.9-17.3), 101.3/105 29.3-339.28). When comparing indicators different groups, statistically significant differences were established (p<0.001, p=0.006). levels population significantly higher than among cancer (median 75.6 4.4, median 317.3 101.3, respectively). data obtained demonstrate changes T- B-cell lymphopoiesis Quantitative determination makes it possible assess state link immune system neoplasms.